ZP3 Antibody (G-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 23-322 mapping within an extracellular domain of ZP3 of human origin
ZP3 Antibody (G-1) is recommended for detection of ZP3 of mouse, rat and human origin by WB, IP, IF and ELISA
ZP3 Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ZP3 (G-1): sc-398359.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The mammalian zona pellucida is composed of three major glycoproteins, ZP1, ZP2 and ZP3. ZP2 has been implicated as a secondary sperm receptor that binds sperm only after the induction of the sperm acrosome reaction. Both ZP2 and ZP3 are modified by the zona reaction; ZP2 undergoes a proteolytic cleavage and ZP3 loses its ability to induce the acrosome reaction and its sperm receptor activity. During the process of fertilization, the initial interaction between male and female gametes is mediated by a sperm receptor, ZP3, which resides in the extracellular glycoprotein matrix (zona pellucida) surrounding the oocyte. The sperm receptor function of the ZP3 molecule plays a key role in the first step of the fertilization process. Following sperm-oocyte binding, ZP3 triggers the sperm acrosome reaction that releases the protein machinery, enabling a spermatozoon to penetrate the zona pellucida.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Does this antibody (ZP3) need permeabilization solution(Triton X-100) ?
Asked by: Ali reza 62
Thank you for your question. Yes, if fixing with formalin or PFA then we do recommend permeablization with Triton X-100. A link to our complete recommended protocol for IF is below as a reference.
Answered by: Technical Support
Date published: 2018-12-10
I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-398359: ZP3 (G-1) mouse monoclonal antibody will work?
Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Rated 5 out of
Date published: 2017-10-07
Rated 5 out of
Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-07
Rated 5 out of
Nice Western blot data of ZP3 expressionNice Western blot data of ZP3 expression in human ovary and human placenta tissue extracts. -SCBT QC
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