ZP3 Antibody (G-1)recommended for detection of ZP3 of mouse, rat and human origin by WB, IP, IF and ELISA

ZP3 Antibody (G-1): sc-398359

ZP3 Antibody (G-1) is rated 5.0 out of 5 by 3.
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Datasheets

    • ZP3 Antibody (G-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 23-322 mapping within an extracellular domain of ZP3 of human origin
    • ZP3 Antibody (G-1) is recommended for detection of ZP3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • ZP3 Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ZP3 (G-1): sc-398359.
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Does this antibody (ZP3) need permeabilization solution(Triton X-100) ?

Asked by: Ali reza 62
Thank you for your question. Yes, if fixing with formalin or PFA then we do recommend permeablization with Triton X-100. A link to our complete recommended protocol for IF is below as a reference. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-12-10

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-398359: ZP3 (G-1) mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from 抗体很好用 我一个月前购买了此产品,做荧光,很特异,做western曝光也很好,3秒就可以出来,而且价钱相对便宜
Date published: 2017-10-07
Rated 5 out of 5 by from Reliable Antibody, Great for WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-07
Rated 5 out of 5 by from Nice Western blot data of ZP3 expression Nice Western blot data of ZP3 expression in human ovary and human placenta tissue extracts. -SCBT QC
Date published: 2013-01-04
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