Anti-ZO-1 Antibody (R40.76) recommended for detection of ZO-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-ZO-1 Antibody (R40.76): sc-33725

Anti-ZO-1 Antibody (R40.76) is rated 4.0 out of 5 by 45.
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Datasheets

    • Anti-ZO-1 Antibody (R40.76) is a rat monoclonal IgG2a ZO-1 antibody, cited in 128 publications, provided at 200 µg/ml
    • raised against DOC-insoluble junctional ribbons isolated from rat liver
    • Anti-ZO-1 Antibody (R40.76) is recommended for detection of ZO-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-ZO-1 Antibody (R40.76) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ZO-1 (R40.76): sc-33725.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ZO-1 Antibody (R40.76). This work is in progress.
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我想问一下ZO-1一抗有小鼠的吗

Asked by: liang32
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Hi there, I would like to ask about ZO-1 Antibody (R40.76): sc-33725. Does this antibody detect both ZO-1α- and ZO-1α+ isoforms? or one of these isoforms? Many thanks!

Asked by: Shoma0308
For best assistance contact Technical Service.
Answered by: Tech Sales
Date published: 2021-07-09

which do you have any recommended zo-1 isotype antigen(negative control)?

Asked by: nonono
Thank you for your question. ZO-1 Antibody (R40.76) is a rat monoclonal IgG2a. The recommended isotype negative control is normal rat IgG2a: sc-3883.
Answered by: Technical Support
Date published: 2019-03-15

Can you please send our lab a FREE 10 µg sample of ZO-1 (R40.76): sc-33725. Seema Bhalchandra Division of Geo Med/ID, Tupper 525 15, Kneeland Street Tufts Medical Center Boston, MA02111

Asked by: Seema
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

请问为什么这个蛋白杂出来的结果又多个条带?是因为这个蛋白本身有很多条带还是因为抗体的非特异呢?求解 谢谢。

Asked by: Anonymous
感谢您的提问。关于结果条带的问题,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to check the details for you. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-06-15

do you have a secondary antibody: anti-rat? What do I use for detection of ZO-1 (R40.76): sc-33725.?

Asked by: sofiavalles
Thank you for your question. At this time we do not have an anti-rat secondary though we are working on developing an appropriate detection reagent. Any commercial anti-rat secondary will be reactive with our ZO-1 sc-33725.
Answered by: Technical Service
Date published: 2019-09-09

我想问一下occludin和ZO-1的抗体用于IF,是用于冰冻切片还是石蜡切片的?

Asked by: 宝啦啦啦啦
感谢您的提问。ZO-1 抗体 (R40.76): sc-33725既可以用于冰冻切片,也可用于石蜡切片。抗体的推荐实验方法中有IF,代表此抗体可用于细胞爬片和冰冻切片的免疫荧光和免疫组化;有IHC(P) ,代表抗体可用于石蜡切片的免疫荧光和免疫组化。更多问题,欢迎通过:电话(+86) 021-60936351、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. ZO-1 antibody (R40.76): sc-33725 is applicable to both frozen tissue and paraffin-embedded tissue. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936351 or [email protected] or by live chat on our website. Best of luck with your research!
Answered by: Technical Support 14
Date published: 2017-04-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 4 out of 5 by from 功能很好 如果能领到会很开心,这个抗体听说很好用,准备买这个,但可以先领用试用装,服务挺好的公司,非常值得大家使用
Date published: 2021-12-24
Rated 4 out of 5 by from 到货及时,功能突出 上海圣克鲁斯厂家沟通起来非常顺畅,邮件回复很详细耐心,根据客户要求及时查货,订单确认后及时发货,实验结果也较为理想。
Date published: 2021-07-28
Rated 4 out of 5 by from work well on frozen tissue IF we tested it on WB, Frozen tissue IF, and IHC-P. It worked well on frozen tissue IF, not that good on WB and IHC-P.
Date published: 2020-12-21
Rated 1 out of 5 by from scarso comprato alcuni mesi fa, testato su linee cellulari, aspecifici da non consentire una corretta individuazione della proteina di interesse
Date published: 2019-11-25
Rated 1 out of 5 by from poor quality I have tried this antibody in a murine lung endothelial cell line detecting a faint 'smir' by immunoblot, similar to the one detected in HT22 neurons. Moreover, I have tried it in mouse brain detecting not signal at all
Date published: 2019-04-19
Rated 4 out of 5 by from Good antibody It can be used to detect ZO-1 in MDA‐MB‐231 cell in western blotting.
Date published: 2019-03-19
Rated 4 out of 5 by from The antibody is good The antibody was used to examine protein of ZO-1 by western blotting method. it could be used in human tissues.
Date published: 2018-11-14
Rated 1 out of 5 by from did not work in IF this antibody did not work in IF for Caco-2 cells.
Date published: 2018-05-10
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