ZnT-8 Antibody (B-9)recommended for detection of ZnT-8 of mouse, rat, human origin by WB, IP, IF and ELISA

ZnT-8 Antibody (B-9): sc-514715

ZnT-8 Antibody (B-9) is rated 5.0 out of 5 by 2.
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Datasheets

    • ZnT-8 Antibody (B-9) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-79 mapping at the N-terminus of ZnT-8 of mouse origin
    • ZnT-8 Antibody (B-9) is recommended for detection of ZnT-8 of mouse, rat and human origin by WB, IP, IF and ELISA
    • ZnT-8 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ZnT-8 (B-9): sc-514715.
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Are there any issues with using sc-514715: ZnT-8 (B-9) mouse monoclonal primary antibody on mouse samples?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-514715 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Strong Western blot signal in a mouse cell line It was the first time that I used this antibody for Western blot, and it can detect a very strong band (about 55 kDa) in a mouse cell line. The size is much bigger than I expected. I will verify the antibody's specificity in my future experiment. I will try whether it will also work in human cells as the SCBT datasheet described.
Date published: 2018-11-09
Rated 5 out of 5 by from Exellent for my ICC staining A few days ago, I did immunofluorescence using this antibody in my working cell line (I don't want to mention that cell line here before our manuscript get accepted), and this antibody works great. This antibody is very specific with extremely low backgroud. I will highly recommed it to my friends and colleugues. I will update my review when I get Western blot results next week.
Date published: 2018-05-22
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