ZBP1 Antibody (H-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 1-300 mapping at the N-terminus of ZBP1 of mouse origin
ZBP1 Antibody (H-9) is recommended for detection of ZBP1 of mouse, rat and human origin by WB, IP, IF and ELISA
ZBP1 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for ChIP application (sc-271483 X, 200 µg/0.1 ml)
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ZBP1 (H-9): sc-271483.
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Left-handed Z-DNA is a higher energy form of the double helix. Proteins containing Za domains share a remarkable ability to bind specifically to Z-DNA and/or Z-RNA. ZBP1 (Z-DNA binding protein 1), also designated DLM-1, is a 429 amino acid protein that harbors two copies of the Za domain containing the Za motif at its N-terminus. ZBP1 is involved in host responses against cellular stresses, including tumorigenesis and viral infection. It is highly expressed in lymphatic tissues including leukocytes, lymph node, tonsil, bone marrow, spleen and, to a lesser extent, in thymus, lung and liver. There are five known isoforms of human ZBP1. The ZBP1 protein shares 47% and 46% sequence identity with the mouse and rat homologs, respectively. The mouse, rat, and human ZBP1 proteins all contain four conserved regions, two of which are homologous to the Z-DNA binding domains Za and Zb of the RNA editing enzyme ADAR1.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
The bands are running much higher then the predicted 46KD. Have you confirmed with ZBP1-/- mice or siRNA to show specificity?
Asked by: Hskoehle
Thank you for your question. While the predicted weight is 46 kDa based on sequence, the published weights for ZBP1 range as high as 68 kDa. We do not have any KO data for this target at this time. Please contact Technical Service by phone, (800)-457-3801 option 2, email <email@example.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-06-27
I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-271483: ZBP1 (H-9) mouse monoclonal antibody will work?
Asked by: Trav11
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
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