Anti-YAP1 Antibody (G-6) recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-YAP1 Antibody (G-6): sc-376830

Anti-YAP1 Antibody (G-6) is rated 4.7 out of 5 by 29.
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Datasheets

    • Anti-YAP1 Antibody (G-6) is a mouse monoclonal IgG1 κ YAP1 antibody, cited in 55 publications, provided at 200 µg/ml
    • raised against amino acids 206-330 mapping near the C-terminus of YAP of human origin
    • Anti-YAP1 Antibody (G-6) is recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-YAP1 Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-376830 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of YAP1 (G-6): sc-376830.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for YAP1 Antibody (G-6) for WB and IHC(P) applications. These reagents are now offered in bundles with YAP1 Antibody (G-6) (see ordering information below).
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what is the certain antibody for rabbit YAP WB test (anti-YAP (1 : 1000; Santa Cruz, USA) in the study <Hydrostatic Pressure Modulates Intervertebral Disc Cell Survival and Extracellular Matrix Homeostasis via Regulating Hippo-YAP/TAZ Pathway>?

Asked by: Markhu
Thank you for your question. We don't know the certain antibody for YAP about this paper. If the author writes both clone number and protein name, then we can know the specific antibody. For YAP, we provide related products, please check the link below. https://www.scbt.com/search?Ntt=YAP1 感谢提问。 这篇文章中,我们并不知道,作者具体使用了我司哪个货号的抗体。 只有当作者同时写明了抗体的克隆号和蛋白名/基因名时,我们才知道具体的货号。 关于YAP,我们公司有如下的相关产品,请查看链接。 https://www.scbt.com/search?Ntt=YAP1
Answered by: Ling Chen
Date published: 2022-07-07

请问这个抗体是可以检测不同亚型的蛋白吗?他们的kd值是否有明显差别?

Asked by: grazylee
Thanks for your question. Anti-YAP1 Antibody (G-6) is recommended for detection of total YAP. Molecular Weight of YAP: 65 kDa. 感谢您的提问。Anti-YAP1 抗体(G-6) 推荐检测总 YAP ,YAP分子量是65kDa。
Answered by: Yanru Tao
Date published: 2022-06-09

Can this antibody be used in huaman BMMSCS samples in WB?

Asked by: AngelWang
Thank you for your question. We have not tested this antibody specifically on Bone Marrow Mesenchymal Stem Cells, so I am unable to specifically say if the YAP protein is expressed in these cells. We recommend you research in the primary literature to see if these BMMSCS cells express the YAP protein.
Answered by: Tech Service
Date published: 2019-12-02

Has anyone tried this anybody for ChIP? Our lab used the predecessor antibody (now discontinued) for ChIP and I need to do it again looking for some other binding sites.

Asked by: Hope34Me
Thank you for your question. We are not currently aware of any data or product citations using this antibody for ChIP research applications. We do offer an alternative antibody, YAP Antibody (63.7): sc-101199, which has been published in ChIP research applications.
Answered by: Technical Support
Date published: 2019-06-16

Is antigen retrieval recommended for this antibody in IHC? If so, are there any guidelines about which antigen retrieval methods are best for the protein?

Asked by: annag16
Thank you for your question. We recommend antigen retrieval for this antibody, sodium citrate with heat or EDTA are the preferred methods. You can find the recommended IHC protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-01-14

Hello, I'm interested in getting  a FREE 10 µg sample of YAP (G-6): sc-376830. We are working with the mouse intestinal organoids and would like to detect how YAP protein localisation changes during oncoprotein activation. I would appreciate your reply.

Asked by: hui99
Thank you for your question. If you have an interest in testing a free 10 µg sample of sc-376830, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Can this mouse monoclonal antibody be used to stain mouse samples in IF and IHC?

Asked by: AbPolly
Thank you for your question. Yes, this antibody is recommended for use in IF and IHC with mouse samples. We recommend using a directly conjugated version of the antibody. This YAP antibody, sc-376830, is available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 14
Date published: 2017-01-20
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Rated 4 out of 5 by from Good. YAP antibody detected reasonably in human stromal cells by WB, dilution 1:500.
Date published: 2022-04-18
Rated 5 out of 5 by from Great antibody! It works well in immunofluorescence. 1:100 dilution
Date published: 2021-10-21
Rated 1 out of 5 by from the batch A2220 does not work at all! the batch A2220 does not work at all! Gives a band at the wrong size lower than 60Kda, and a strong laddering above. Another batch of the same ref did work with my samples.
Date published: 2020-12-10
Rated 5 out of 5 by from Good at WB/IF Nice antibody for Immunofluorescence and western blotting!
Date published: 2020-11-30
Rated 4 out of 5 by from 功能很出色 我一个月前购买此产品,并为此感到高兴,之后有需要的话会再购买,主要是抗体浓度需要提高到很高希望能在这方面加以改善,谢谢
Date published: 2020-10-22
Rated 5 out of 5 by from Nice and single band (w.b.) Produced a single band at the expected molecular weight (~65kDa). Sample: MDA-MB-231 wcl (30ug). Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer, overnight @ 4oC. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 30 seconds* *further dilution of the primary antibody (1:400-1:500 ?) may yield similar results at the expense of a prolonged exposure time (2-4 minutes).
Date published: 2017-11-18
Rated 5 out of 5 by from Good antibody! I used this antibody for WB. I detected a band at 65 kDa. I'm satisfied with the antibody.
Date published: 2017-11-06
Rated 5 out of 5 by from Good antibody! 1:500 diluted for wb of rat heart protein,the band is strong and special.
Date published: 2017-11-01
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