Anti-YAP1 Antibody (B-8) recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-YAP1 Antibody (B-8): sc-398182

Anti-YAP1 Antibody (B-8) is rated 5.0 out of 5 by 3.
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Datasheets

    • YAP1 Antibody (B-8) is a mouse monoclonal IgG2a κ, cited in 8 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 294-323 within an internal region of YAP of human origin
    • Anti-YAP1 Antibody (B-8) is recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-YAP1 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-398182 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of YAP1 (B-8): sc-398182.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for YAP1 Antibody (B-8) for WB applications. These reagents are now offered in bundles with YAP1 Antibody (B-8) (see ordering information below).
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请问这个抗体检测的是全长的YAP还是截短了的YAP。如果是截短的,那截掉的是哪段?

Asked by: Xiamensc
感谢您的提问。YAP 抗体 (B-8): sc-398182针对的特异性抗原位点位于人源YAP内部区域内的氨基酸294-323之间。更多问题,欢迎通过:电话(+86) 021-60936351、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. YAP antibody (B-8): sc-398182 is specific for an epitope mapping between amino acids 294-323 within an internal region of YAP of human origin. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936351 or [email protected] or by live chat on our website.
Answered by: Tech Service
Date published: 2017-03-27

When should the TransCruz reagent sc-398182 X be used instead of the standard form sc-398182?

Asked by: jenniferc
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 15
Date published: 2017-01-19
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Rated 5 out of 5 by from Superb product Very nice western blot results; strong signal with low background
Date published: 2017-02-01
Rated 5 out of 5 by from Excellent! This antibody works great in Western blot analysis of YAP expression in non-transfected 293T: sc-117752, human YAP transfected 293T: sc-115429 and Caco-2 whole cell lysates.
Date published: 2017-01-11
Rated 5 out of 5 by from Great for Western Blot Great for Western Blot, can detect both endogenous and transfected YAP in cell lysates -SCBT QC
Date published: 2013-07-30
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