Anti-XAF1 Antibody (B-5) recommended for detection of XAF1 of human origin by WB, IP, IF and ELISA

Anti-XAF1 Antibody (B-5): sc-398012

Anti-XAF1 Antibody (B-5) is rated 5.0 out of 5 by 1.
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Datasheets

    • XAF1 Antibody (B-5) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-225 mapping at the N-terminus of XAF1 of human origin
    • recommended for detection of XAF1 of human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for XAF1 Antibody (B-5) for WB applications. These reagents are now offered in bundles with XAF1 Antibody (B-5) (see ordering information below).

What is the recommended starting dilution for Western blot for sc-398012: XAF1 (B-5) monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Nice Western Blot data of XAF1 expression in non Nice Western Blot data of XAF1 expression in non-transfected and human XAF1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-05-09
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