Anti-WISP-1 Antibody (A-9) recommended for detection of WISP-1 of human origin by WB, IP, IF and ELISA

Anti-WISP-1 Antibody (A-9): sc-133126

Anti-WISP-1 Antibody (A-9) is rated 4.0 out of 5 by 6.
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Datasheets

    • Anti-WISP-1 Antibody (A-9) is a mouse monoclonal IgG2b κ WISP-1 antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 311-367 of WISP-1 of human origin
    • WISP-1 Antibody (A-9) is recommended for detection of WISP-1 of human origin by WB, IP, IF and ELISA
    • Anti-WISP-1 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of WISP-1 (A-9): sc-133126.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for WISP-1 Antibody (A-9) for WB applications. These reagents are now offered in bundles with WISP-1 Antibody (A-9) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with WISP-1 (A-9): sc-133126 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from localiza la forma activa (dímeros) La proteína tiene un tamaño aproximado de 70 kDa, por lo que el anticuerpo localiza dímeros de WISP1.
Date published: 2017-06-20
Rated 4 out of 5 by from good antibody! wisp-1 was detected in ESCC cell lines with a little background.
Date published: 2017-05-08
Rated 1 out of 5 by from No specific WISP1 bands were seen No specific WISP1 bands were seen - tested human glioma stem cells.
Date published: 2015-04-14
Rated 5 out of 5 by from Good WB data gathered in K Good WB data gathered in K-562, Caco-2, Hep G2 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Beautiful IF staining with Hep G2 samples Beautiful IF staining with Hep G2 samples. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by from Good data for WB in recombinant WISP Good data for WB in recombinant WISP-1. -SCBT QC
Date published: 2014-09-10
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