WDR79 Antibody (1F12)recommended for detection of WDR79 of human origin by WB, IP, IF and ELISA

WDR79 Antibody (1F12): sc-517078

WDR79 Antibody (1F12) is rated 5.0 out of 5 by 1.
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Datasheets

    • WDR79 Antibody (1F12) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against amino acids 62-160 representing partial length WDR79 of human origin
    • recommended for detection of WDR79 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of WDR79 (1F12): sc-517078.
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I am using WDR79 (1F12): sc-517078 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Works for WB/IF/PLA Bought this antibody (WDR79) to measure protein-protein interactions (amongst other things) in 293T/HeLa lysates. Worked great! WB 1:1000 and IF/PLA 1:100. Really helpful in small molecule assays! Would definitely use again.
Date published: 2017-08-15
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