WDR61 Antibody (WW-5)recommended for detection of WDR61 of human origin by WB, IP, IF and ELISA

WDR61 Antibody (WW-5): sc-100897

WDR61 Antibody (WW-5) is rated 3.7 out of 5 by 3.
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Datasheets

    • WDR61 Antibody (WW-5) is a mouse monoclonal IgG2b (kappa light chain) provided at 100 µg/ml
    • raised against recombinant WDR61 of human origin
    • recommended for detection of WDR61 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of WDR61 (WW-5): sc-100897.
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I am using WDR61 (WW-5): sc-100897 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from quite good Quite ok on human lymphoblastoid cell lysate at 1/1000. Few aspecificities. Should by better at 1/800
Date published: 2018-08-31
Rated 4 out of 5 by from Good specificity in WB in HeLa whole cell lysate Good specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-04
Rated 4 out of 5 by from Produced robust immunofluorescence nuclear Produced robust immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-06
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