WDR1 Antibody (B-10)recommended for detection of WDR1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

WDR1 Antibody (B-10): sc-393159

WDR1 Antibody (B-10) is rated 4.8 out of 5 by 4.
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Datasheets

    • WDR1 Antibody (B-10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 425-606 mapping at the C-terminus of WDR1 of human origin
    • WDR1 Antibody (B-10) is recommended for detection of WDR1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • WDR1 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of WDR1 (B-10): sc-393159.
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I am using WDR1 (B-10): sc-393159 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good clean antibody I received this antibody as a free sample and fortunately it worked on the first try. I used it at 1:500 as the sample given only had 50 ul. The recommended 1:100 would be 100 ul for 10 ml. Using their anti-Igkappa-HRP, it gave clean bands. See figure legend for details.
Date published: 2017-10-16
Rated 4 out of 5 by from It is a very good antibody! The quality is very good, but I was dissapointed to find out that I did not receive the quantity written on the vial. On the vial it is written 10micrograms in 50 microL, but I only received 20microL. I was not enough for all my samples. Another colleague of mine also received 30 microL instead od 50microL.
Date published: 2017-09-18
Rated 5 out of 5 by from STRONG BAND In WESTERN I got the sample antibody and tried it in both HT-29 and HT-22 cell lysates. Both show strong bands.
Date published: 2016-11-14
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2015-02-08
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