Anti-WAVE2 Antibody (C-6) recommended for detection of WAVE2 of mouse, rat, human and origin by WB, IP, IF and ELISA

Anti-WAVE2 Antibody (C-6): sc-373889

Anti-WAVE2 Antibody (C-6) is rated 4.2 out of 5 by 11.
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Datasheets

    • Anti-WAVE2 Antibody (C-6) is a mouse monoclonal IgM κ WAVE2 antibody, cited in 4 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 177-206 within an internal region of WAVE2 of human origin
    • recommended for detection of WAVE2 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of WAVE2 (C-6): sc-373889.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for WAVE2 Antibody (C-6) for WB applications. This reagent is now offered in a bundle with WAVE2 Antibody (C-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What application is the blocking peptide sc-373889 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from WAVE2 Antibodies (C-6): sc-373889 work in WB and a Image shows analysis of WAVE2 level in LN229 cells upon CRISPR-Cas9-mediated KO WAVE2 using two different gRNAs. WAVE2 Antibodies (C-6): sc-373889 detect a band of approximately 84 kDa (corresponding to molecular weight of WAVE2) and are specific.
Date published: 2020-07-31
Rated 1 out of 5 by from Does not work! Used for WB with a 1:300 dilution in SUM159, A7r5, Caco-2 and 293T cell lysates. No signal at all.
Date published: 2017-11-15
Rated 1 out of 5 by from No banding in range There were no detectable bands within the expected range in U2OS cells.
Date published: 2017-07-31
Rated 5 out of 5 by from Western blot Western blot we obtained a right band arount 90kda, 1:600 dilution for WB.
Date published: 2017-05-28
Rated 5 out of 5 by from specific band Showing only one specific band on western blot for HepG2 cell. Dilution is 1:1000.
Date published: 2017-05-28
Rated 5 out of 5 by from specific bands The specific bands were detected by Western blot and 1:500 in mouse tissues,strong target band at 90kda.
Date published: 2017-05-27
Rated 5 out of 5 by from Good product. The analysis results of Western blot were very strong and clear.Good product.
Date published: 2017-04-28
Rated 5 out of 5 by from Good antibody for IF I used to work with the H-110 clone but as it has been discontinued, I asked for a free sample of this antibody. The picture presented on the website was not highly convincing as WAVE2 should be enriched at the leading edge. Although, I was really surprised because I have got a very good localisation in my cells. I have tested both antibodies in parallel and for the same dilution (1:50), signal was weaker for the C-6 clone but much cleaner.
Date published: 2017-03-15
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