VPS53 Antibody (H-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 170-343 mapping within an internal region of VPS53 of human origin
VPS53 Antibody (H-4) is recommended for detection of VPS53 of mouse, rat and human origin by WB, IP, IF and ELISA
VPS53 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VPS53 (H-4): sc-514920.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Vacuolar sorting proteins (VPSs) are required for proper trafficking of endocytic and biosynthetic proteins to the vacuole and play an important role in the budding process of cells. VPS53 (vacuolar protein sorting 53), also known as PP13624 or HCCS1, is a 699 amino acid protein that localizes to both the golgi apparatus and the endosome membrane and belongs to the VPS family. Expressed as multiple alternatively spliced isoforms, VPS53 functions as a component of the multi-protein golgi-associated retrograde protein (GARP) complex and is thought to be involved in retrograde transport of early and late endosomes to the golgi. The gene encoding VPS53 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What blocking solution should I use with VPS53 (H-4): sc-514920 monoclonal antibody for Western blot?
Asked by: Randy McDonald
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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