VPS4A Antibody (A-11)recommended for detection of VPS4A of mouse, rat and human origin by WB, IP, IF and ELISA

VPS4A Antibody (A-11): sc-393428

VPS4A Antibody (A-11) is rated 4.6 out of 5 by 5.
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Datasheets
    • VPS4A Antibody (A-11) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 349-370 near the C-terminus of VPS4A of human origin
    • VPS4A Antibody (A-11) is recommended for detection of VPS4A of mouse, rat and human origin by WB, IP, IF and ELISA
    • VPS4A Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-393428 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VPS4A (A-11): sc-393428.
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What application is the blocking peptide sc-393428 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from a lot of unspecific bands I've tested this antibody in parallel with a polyclonal antibody from another company in human fibroblasts. A lot of unspecific signals were observed for the VPS4A A-11 antibody. If you know which is the specific band, its fine. In IF it only shows a cytosolic signal.
Date published: 2018-01-23
Rated 5 out of 5 by from Very good antibody I used this antibody. It works very well and specific. There is no unspecific band.
Date published: 2017-09-06
Rated 5 out of 5 by from Excellent choice for VPS4A detection! This VPS4A mono is a great choice, especially if you are looking for a solid antibody for Western blot. Great choice!
Date published: 2017-02-03
Rated 5 out of 5 by from Nice Western Blot data of VPS4 expression Nice Western Blot data of VPS4 expression in Hep G2 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Good Western Blot data of VPS4A expression in non Good Western Blot data of VPS4A expression in non-transfected 293T, human VPS4A transfected 293T and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-04-16
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