Anti-VPS39 Antibody (C-5) is a mouse monoclonal IgG2a (kappa light chain) VPS39 antibody provided at 200 µg/ml
raised against amino acids 576-875 mapping at the C-terminus of VPS39 of human origin
VPS39 Antibody (C-5) is recommended for detection of VPS39 of human origin by WB, IP, IF and ELISA
Anti-VPS39 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VPS39 (C-5): sc-514762.
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VPS39 Antibody (C-5) is a high quality monoclonal VPS39 antibody (also designated VPS39 antibody) suitable for the detection of the VPS39 protein of human origin. VPS39 Antibody (C-5) is available as both the non-conjugated anti-VPS39 antibody form, as well as multiple conjugated forms of anti-VPS39 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Vacuolar sorting proteins (VPSs) are required for proper trafficking of endocytic and biosynthetic proteins to the vacuole and play an important role in the budding process of cells. VPS39 (vacuolar protein sorting 39), also known as VAM6 or TLP, is an 886 amino acid protein that localizes to the cytoplasm, as well as to the lysosomal and endosomal membrane, and contains one CNH domain. Expressed ubiquitously with highest expression in kidney, heart, lung, brain, placenta and skeletal muscle, VPS39 functions as a homooligomer that is thought to play a role in the clustering and fusion of endosomes and lysosomes. Multiple isoforms of VPS39 exist due to alternative splicing events. The gene encoding VPS39 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-514762: VPS39 (C-5) monoclonal antibody?
Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
Rated 2 out of
Doesnt work with rat samples.Took a chance that it might work on rat VPS39. Antibody was tried at both recommended concentration (datasheet) as well as 1:50 on rat cell line lysates that probed positive for other proteins, no signal seen with high sensitivity HRP reagent.
Date published: 2019-05-24
Rated 2 out of
Antibody titer is lowerThe vps39 antibody titer is very low. I can obtain lane by super ECL at 1:50 dilution.
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