VPS39 Antibody (C-5)recommended for detection of VPS39 of human origin by WB, IP, IF and ELISA

VPS39 Antibody (C-5): sc-514762

VPS39 Antibody (C-5) is rated 2.0 out of 5 by 2.
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Datasheets
    • VPS39 Antibody (C-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 576-875 mapping at the C-terminus of VPS39 of human origin
    • VPS39 Antibody (C-5) is recommended for detection of VPS39 of human origin by WB, IP, IF and ELISA
    • VPS39 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VPS39 (C-5): sc-514762.
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What is the recommended starting dilution for Western blot for sc-514762: VPS39 (C-5) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 2 out of 5 by from Doesnt work with rat samples. Took a chance that it might work on rat VPS39. Antibody was tried at both recommended concentration (datasheet) as well as 1:50 on rat cell line lysates that probed positive for other proteins, no signal seen with high sensitivity HRP reagent.
Date published: 2019-05-24
Rated 2 out of 5 by from Antibody titer is lower The vps39 antibody titer is very low. I can obtain lane by super ECL at 1:50 dilution.
Date published: 2017-06-22
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