VPS37A Antibody (G-3)recommended for detection of VPS37A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

VPS37A Antibody (G-3): sc-376978

VPS37A Antibody (G-3) is rated 4.8 out of 5 by 5.
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Datasheets

    • VPS37A Antibody (G-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 81-380 mapping within an internal region of VPS37A of human origin
    • VPS37A Antibody (G-3) is recommended for detection of VPS37A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • VPS37A Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VPS37A (G-3): sc-376978.
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Can VPS37A (G-3): sc-376978 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, VPS37A (G-3): sc-376978 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from WB detection The antibody shows strong sensitivity for WB analyisis, I got strong signal band used the rat kidney tissue lysates.
Date published: 2017-06-24
Rated 4 out of 5 by from Decent antibody Not sure how sensitive this antibody is, but it seems specific. We could easily detect endogenous VPS37A in HEK293T cells using an HRP-conjugated secondary (1 min exposure). Tubulin was detected with 488-conjugated antibody using a Typhoon TRIO imager.
Date published: 2016-12-16
Rated 5 out of 5 by from Good specificity in WB in MDA Good specificity in WB in MDA-MB-468, BT-20 and SK-BR-3 nuclear extracts. -SCBT QC
Date published: 2015-02-05
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-01-27
Rated 5 out of 5 by from Positive signal in WB in MCF7 nuclear extract Positive signal in WB in MCF7 nuclear extract. -SCBT QC
Date published: 2015-01-04
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