VPS35 Antibody (B-5)recommended for detection of VPS35 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

VPS35 Antibody (B-5): sc-374372

VPS35 Antibody (B-5) is rated 5.0 out of 5 by 16.
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Datasheets
    • VPS35 Antibody (B-5) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 497-796 mapping at the C-terminus of VPS35 of human origin
    • VPS35 Antibody (B-5) is recommended for detection of VPS35 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • VPS35 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VPS35 (B-5): sc-374372.
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What is the recommended secondary reagent to use with VPS35 Antibody (B-5): sc-374372?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-09
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Rated 5 out of 5 by from Works very well for WB Single immunoreactive band at the expected MW on lysates of Jurkat T cells.
Date published: 2019-01-22
Rated 5 out of 5 by from Works very well! I tested this antibody by western blot and ICC on human iPSC derived neurons and it works very well !
Date published: 2018-06-25
Rated 5 out of 5 by from very good! It's work well at the dilotion of 1:1000 in western blot.The band is quite clear.
Date published: 2018-04-04
Rated 5 out of 5 by from VPS35 Antikörper (B-5) Works really good in IF, 1:100 dilution. Nice Colocalization with other retromer components. I have tested so far only human lung fibroblasts.
Date published: 2018-01-23
Rated 5 out of 5 by from Good western blotting antibody excellent single band from human cell lysates at 1/1000
Date published: 2017-09-20
Rated 5 out of 5 by from Excellent antibody N2A total sample was probed with this antibody (1:1000). Thermo regularly strength solution was used for detection.
Date published: 2017-08-11
Rated 5 out of 5 by from recommendation of this antibody I have used this VPS35 antibody in WB and immnofluorescence and recieved great results. I recommend this antibody!
Date published: 2017-04-27
Rated 5 out of 5 by from Great VPS35 antibody Specific, reliable detection of VPS35 confirmed with CRISPR knockout data. Great for mouse, rat, or human detection.
Date published: 2017-01-20
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