VPS25 Antibody (B-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 1-176 representing full length VPS25 of human origin
VPS25 Antibody (B-4) is recommended for detection of VPS25 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
VPS25 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VPS25 (B-4): sc-271648.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Vacuolar sorting proteins (VPSs) are required for trafficking normal endocytic and biosynthetic proteins to the vacuole and also play an important role in the budding process of cells. VPS25 is a highly conserved and widely expressed eukaryotic protein, with single orthologs in chromalveolate, excavate, amoebozoan, plant, fungal and metazoan species. VPS25, along with Vps22p and Vps36p, functions as a subunit of ESCRT-II, an endosomal sorting complex required for protein transport. This complex is essential for the sorting of ubiquitinated biosynthetic and endosomal proteins into endosomes. ESCRT-II transiently associates with the endosomal membrane, thereby initiating the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of mulitvesicular body cargo.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences