VPS16 Antibody (2F10)recommended for detection of VPS16 of human origin by WB, IP, IF and ELISA

VPS16 Antibody (2F10): sc-293327

VPS16 Antibody (2F10) is rated 4.0 out of 5 by 1.
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Datasheets

    • VPS16 Antibody (2F10) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against amino acids 754-839 of VPS16 of human origin
    • recommended for detection of VPS16 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using VPS16 (2F10): sc-293327 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from COMMENTS Good antibody, high specificity and sharp band in WB, 293T lysates, 1:1000 diluted.
Date published: 2017-02-13
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