Anti-VP16 Antibody (14-5) recommended for detection of VP16 and VP16 fusion proteins by WB, IP and IF

Anti-VP16 Antibody (14-5): sc-7546

Anti-VP16 Antibody (14-5) is rated 4.9 out of 5 by 7.
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Datasheets

    • VP16 Antibody (14-5) is a mouse monoclonal IgG2a κ, cited in 28 publications, provided at 200 µg/ml
    • raised against amino acids 410-456 at the N-terminal transcription activation domain of VP16
    • VP16 Antibody (14-5) is recommended for detection of VP16 and VP16 fusion proteins by WB, IP and IF
    • Anti-VP16 Antibody (14-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VP16 (14-5): sc-7546.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for VP16 Antibody (14-5) for WB applications. These reagents are now offered in bundles with VP16 Antibody (14-5) (see ordering information below).
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Hi, may I request for the full sequence of the anti-VP16 antibody to verify its binding sequence and downstream mutation potential?

Asked by: Sheena_sing
Thank you for your question. It would be helpful if you could call us directly at 800- 457-3801 option 2, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-04

Will this antibody detect dCas9-VPR (Cas9 activator)?

Asked by: Kumi
Thank you for your question. This mouse monoclonal antibody is recommended for detection of VP16 and VP16 fusion proteins. We have not tested the antibody for detection of dCas9-VPR. If you have the protein sequence for dCas9-VPR, please contact our Technical Service Department and we can assist you with performing sequence analysis using this antibody's antigen to determine potential cross-reactivity with your protein of interest.
Answered by: Technical Support
Date published: 2019-02-25

Hi, I'm looking for an antisera against VP16. Could you please send me a sample of yours for me to test? I'm looking for one against VP-16 expressed transiently in Tobacco ( N. Benthi). I want to test that the antisera will detect this first.

Asked by: YingS
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

Can this antibody be used with cat samples?

Asked by: AbPolly
This antibody can be used with any sample to detect VP16 fusion proteins. For endogenous VP16, please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 3
Date published: 2017-01-12

If I am using sc-7546 for WB, what do you recommend for detection?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-12
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Rated 5 out of 5 by from Strong western data Very good antibody for western blot; strong signal with low background
Date published: 2017-01-17
Rated 5 out of 5 by from Excellent for fusion protein detection. If you're working with a VP16 fusion protein, this antibody will detect it. It delivers strong WB results, but will work great in your IP and IF as well. 12 citations support it!
Date published: 2016-12-23
Rated 4 out of 5 by from WB data obtained was good but we have seen WB data obtained was good but we have seen better with other VP16 antibodies from SCBT.
Date published: 2015-09-28
Rated 5 out of 5 by from Publishable WB data with transfected Hela and 293T Publishable WB data with transfected Hela and 293T, SW-480, and COS7 cells - SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for CoIP on Cos7 and Vero cells - SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by from Successful application of this antibody in Successful application of this antibody in IF and WB using monkey scFvs-VP16 transfected COS7 cells, and scFvs mammalian cells -SCBT Publication Review
Date published: 2015-03-10
Rated 5 out of 5 by from ChIP publishable data with HeLa ChIP publishable data with HeLa, NCI-H226, HCT116, HCT116 DNMT3B KO and DNMT1/DNMT3B DKO cells - SCBT Publication Review
Date published: 2014-03-12
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