Anti-VP16 Antibody (1-21) recommended for detection of VP16 and VP16 fusion proteins by WB, IP and IF

Anti-VP16 Antibody (1-21): sc-7545

Anti-VP16 Antibody (1-21) is rated 4.8 out of 5 by 5.
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Datasheets

    • Anti-VP16 Antibody (1-21) is a mouse monoclonal IgG1 κ VP16 antibody, cited in 102 publications, provided at 200 µg/ml
    • raised against amino acids 456-490 of VP16
    • VP16 Antibody (1-21) is recommended for detection of VP16 and VP16 fusion proteins by WB, IP and IF
    • Anti-VP16 Antibody (1-21) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VP16 (1-21): sc-7545.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for VP16 Antibody (1-21) for WB applications. These reagents are now offered in bundles with VP16 Antibody (1-21) (see ordering information below).
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What is the recommended secondary reagent to use with VP16 Antibody (1-21): sc-7545 for IF?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins (m-IgGκ BP) conjugated to either FITC, PE or a CruzFluor as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 4 out of 5 by from good for WB of HSV1-infected cells worked well (1:1000 dilution) for detection of HSV1 VP16 in infected 293T cell lysates by western blot.
Date published: 2017-11-30
Rated 5 out of 5 by from Excellent for Western blots Gives strong Western blot signals with very little/no background. It helped us see that one of four VP16 fusion proteins was not expressing well after transfection of Drosophila cells.
Date published: 2017-05-23
Rated 5 out of 5 by from Outstanding western data Very strong western blot signal; little to no background
Date published: 2017-01-04
Rated 5 out of 5 by from Great for detecting VP16! A proven detector of VP16 and VP16 fusion proteins. It works well in all standard applications and will provide reliable results with strong and specific signal. This antibody has been published with nearly 70 times.
Date published: 2017-01-03
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