Vigilin Antibody (H-3) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
raised against amino acids 126-290 mapping near the N-terminus of Vigilin of human origin
Vigilin Antibody (H-3) is recommended for detection of Vigilin of mouse, rat and human origin by WB, IP, IF and ELISA
Vigilin Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Vigilin (H-3): sc-271523.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Vigilin, a K homology (KH) protein, is found in the nucleus and cytoplasm of all eukaryotic species. Vigilin contains a unique structure of 14 to 15 consec-utively arranged KH domains, which function to mediate RNA-protein binding. Expression of the gene encoding Vigilin, which maps to chromosome 2q37, is essential for cell viability. Vigilin is active in heterochromatin formation and cytoplasmic mRNA decay, and can be a useful marker for translational activity. The 80S ribosome co-localizes with Vigilin, which interacts with the ribosomal complex through its C-terminal domain, suggesting its role in the link between tRNA-export and the channeled tRNA-cycle on ribosomes. Intra-cellular cholesterol upregulates Vigilin expression, and the protein specifically binds to high density lipoprotein molecules to function in the removal of excess cellular cholesterol.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences