Anti-Flt-1/VEGFR1 Antibody (D-2) recommended for detection of VEGFR1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Flt-1/VEGFR1 Antibody (D-2): sc-271789

Anti-Flt-1/VEGFR1 Antibody (D-2) is rated 3.7 out of 5 by 14.
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Datasheets

    • Anti-Flt-1/VEGFR1 Antibody (D-2) is a mouse monoclonal IgG1 κ Flt-1/VEGFR1 antibody, cited in 20 publications, provided at 200 µg/ml
    • raised against amino acids 23-247 of VEGFR1 of human origin
    • Flt-1/VEGFR1 Antibody (D-2) is recommended for detection of VEGFR1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Flt-1/VEGFR1 Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Flt-1/VEGFR1 (D-2): sc-271789.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Flt-1/VEGFR1 Antibody (D-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with Flt-1/VEGFR1 Antibody (D-2) (see ordering information below).
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Can sc-271789 also detect soluble FLT-1 and do you have links to western blot pictures where it has been used. Thanks

Asked by: Toluwalase
Thank you for your question. According to UniProt, soluble Flt-1 (isoforms 2, 3 and 4) are missing C-terminal portions of this protein. This monoclonal antibody recognizes an epitope that is N-terminal and present in these isoforms, so this antibody should be able to recognize soluble Flt-1. However, we have not specifically tested this antibody for detection of the soluble isoforms, and we can not provide such data on our website at this time. You may find some of the product citations using this antibody helpful to see if other researchers have published this type of data with this antibody.
Answered by: Technical Support
Date published: 2019-07-10

Can Flt-1 (D-2): sc-271789 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Flt-1 (D-2): sc-271789 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from it gave some non-specific bands We used for WB 1:600 and gave some non-specific bands
Date published: 2021-10-29
Rated 1 out of 5 by from does not work we tested the antibody with different concentrations using different cell lysate but we couldn't get a well defined band of the protein
Date published: 2021-07-13
Rated 1 out of 5 by from unfortunately does not works in IHC Paraffin embedded human jejunal sections (n=6; 5 µm) were rehydrated, antigen retrieval was performed heating sections 25 minutes at 90 °C in a water bath in the presence of 10 mmol / l sodium citrate buffer pH 6.0. Sections were treated with an endogenous peroxidase blocking kit. Immunostaining was performed using a commercial kit following the manufacturer's instructions. Ab Dilution: 0.02 µg/ µl In the jejunum (full thickness) of control samples, the antibody does not work. No signal found in any gastrointestinal layer of the n=6 tested samples. Probably a different antigen retrieval method should be used to expose the epitope.
Date published: 2020-07-01
Rated 1 out of 5 by from Did not work for WB analysis Tried at a 1:500 dilution in human and canine bladder cancer cells but detected no signal.
Date published: 2018-06-19
Rated 5 out of 5 by from Gute Ergebnisse IHC-Paraffin Habe den AK 1:100 in Paraffin getestet mit guten Ergebnissen.
Date published: 2018-02-14
Rated 5 out of 5 by from works for WB used for blotting flt-1 transfected cells. worked well!
Date published: 2017-10-03
Rated 1 out of 5 by from IHC Assay Review for Flt-1 mAB Application: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from positive band This antibody is very sensitivity for Flt-1 detecion ,we use Hela cell lysates only loading 30ug, but signal appears very strong and specific.
Date published: 2017-08-12
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