VEGF Antibody (SPM225) recommended for detection of 121, 165 and 189 VEGF isoforms of mouse, rat, human and canine origin by WB, IP, IF and IHC(P)

VEGF Antibody (SPM225): sc-65617

VEGF Antibody (SPM225) is rated 4.6 out of 5 by 5.
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Datasheets

    • VEGF Antibody (SPM225) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant VEGF189 of human origin
    • recommended for detection of 121, 165 and 189 VEGF isoforms of mouse, rat, human and canine origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See VEGF (C-1): sc-7269 for VEGF antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended secondary reagent to use with VEGF (SPM225): sc-65617 mouse monoclonal primary antibody for Western blot?

Asked by: jenni
We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 5 out of 5 by from Very good results Work very well to detect VEGF expression, this antibody signal is strong
Date published: 2017-05-12
Rated 5 out of 5 by from results are satisfactory VEGF Antibody (SPM225):sc-65617 works very well for IHC(P), showing strong nuclear staining pattern.
Date published: 2017-04-19
Rated 4 out of 5 by from Very nice product Very clear western blot but not as good as c-1 clone for most applications
Date published: 2017-01-04
Rated 5 out of 5 by from A good antibody for WB! A good choice for all you WB needs. It has a broad range of reactivity and will provide a strong specific signal. It has been featured in several citations.
Date published: 2017-01-03
Rated 4 out of 5 by from Works well in WB with 3T3 Works well in WB with 3T3-L1 lysates -SCBT Publication Review
Date published: 2015-01-10
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