Vitamin D Receptor/VDR siRNA and shRNA Plasmids (h) human Vitamin D Receptor/VDR-specific siRNA, shRNA Plasmid and Lentiviral Particle gene silencers

Vitamin D Receptor/VDR siRNA and shRNA Plasmids (h)

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Datasheets

    • Target species: human; for corresponding mouse product, see Vitamin D Receptor/VDR siRNA and shRNA Plasmids (m)
    • Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
    • For independent verification of Vitamin D Receptor/VDR gene silencing results, individual duplex components or plasmids are also available upon request
    • Suitable control antibody: Vitamin D Receptor/VDR Antibody (D-6): sc-13133 is recommended as control antibody for monitoring of Vitamin D Receptor/VDR expression knockdown by Western blotting or immunofluorescence
    • For further details describing the si/shRNA Gene Silencers click here
    • Gene silencers are intended for noncommercial and research use only. Click here for details.

Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanVDR742112q13.11XM_006719587, XM_011538720, NM_000376, NM_001017535, NM_001017536P11473601769
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Gentile SantaCruz B., vorrei urilizzare il vostro prodotto sc-106692-V. E' necessario lavorare in una P2 Room? E' possibile ordinare il prodotto da una struttura che non possiede una P2 Room? Il prodotto è efficiente su linee cellulari primarie?

Asked by: Francesco1977
Grazie per la domanda. Le particelle Lentivirali possono essere utilizzate in strutture con una Biosicurezza standard di livello 2 (e devono essere trattate con lo stesso livello di cauzione di ogni altro reagente potenzialmente infettivo) Le particelle Lentivirali sono replicazione-incompetenti e progettate per auto-inattivarsi in seguito alla trasduzione e all'integrazione dei costrutti degli shRNA nel DNA genomico delle cellule target ll laboratorio che richiede il prodotto deve fornirci la documentazione che attesta una biosicurezza di livello 2. Le particelle lentivirali arrivano pronte per essere usate virtualmente con ogni tipo cellulare di mammifero, incluse linee primarie e cellule non-dividing. L'efficienza su cellule primarie dipende fortemente dal tipo cellulare ed è necessario procedere a test, ad ogni modo garantiamo risultati soddisfacenti. Per qualsiasi dubbio o problema contatti pure il supporto tecnico. Thank you for your question. Lentiviral particles can be employed in standard Biosafety Level 2 tissue culture facilities (and should be treated with the same level of caution as with any other potentially infectious reagent). The Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of the shRNA constructs into the genomic DNA of target cells. shRNA Lentiviral Particles arrive ready to add to virtually any mammalian cell type, including primary and non-dividing cells. Efficiency on primary cells strongly depends on the cell line type and you need to test it, anyway we guarantee satisfactory results. For any doubt or problem please contact our technical support.
Answered by: Technical Support Europe
Date published: 2017-02-10
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