VAMP-1/2 Antibody (SP10)recommended for detection of VAMP-1 (amino acids 1-118) and VAMP-2 (amino acids 33-96) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

VAMP-1/2 Antibody (SP10): sc-20039

VAMP-1/2 Antibody (SP10) is rated 4.4 out of 5 by 5.
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Datasheets

    • VAMP-1/2 Antibody (SP10) is a mouse monoclonal mouse IgM κ (kappa light chain) provided at 200 µg/ml
    • raised against synaptic vesicle-containing fractions of immunoprecipitated human brain homogenate
    • recommended for detection of VAMP-1 (amino acids 1-118) and VAMP-2 (amino acids 33-96) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using VAMP-1/2 (SP10): sc-20039 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Well-characterized antibody This monoclonal VAMP-1/2 Antibody (SP10): sc-20039 is a well-characterized clone with published data in IHC(P) and Western blotting and featured in 3 publications in the literature.
Date published: 2017-02-03
Rated 4 out of 5 by from Publishable data in IF using mouse brain Publishable data in IF using mouse brain (cerebral cortex, hippocampus, thalamus, cerebellum -SCBT Publication Review
Date published: 2015-06-17
Rated 4 out of 5 by from Robust band seen in WB using rat brain Robust band seen in WB using rat brain. -SCBT QC
Date published: 2015-05-29
Rated 5 out of 5 by from Produced great immunoperoxidase membrane Produced great immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human epididymis tissue. -SCBT QC
Date published: 2015-04-24
Rated 4 out of 5 by from Strong band seen in WB using mouse brain Strong band seen in WB using mouse brain tissue extract. -SCBT QC
Date published: 2015-04-11
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