Anti-V-ATPase D Antibody (E-12) recommended for detection of V-ATPase D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-V-ATPase D Antibody (E-12): sc-390384

Anti-V-ATPase D Antibody (E-12) is rated 3.7 out of 5 by 3.
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Datasheets

    • Anti-V-ATPase D Antibody (E-12) is a mouse monoclonal IgG1 κ V-ATPase D antibody, cited in 1 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 211-249 near the C-terminus of V-ATPase D of human origin
    • Anti-V-ATPase D Antibody (E-12) is recommended for detection of V-ATPase D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-V-ATPase D Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V-ATPase D (E-12): sc-390384.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for V-ATPase D Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with V-ATPase D Antibody (E-12) (see ordering information below).
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What application is the blocking peptide sc-390384 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 1 out of 5 by from wrong band? I received this antibody as a sample. The antibody did not recognize a 28kDa band as predicted. Instead it recognized a single band at ~250kDa in HEK293 lysate.
Date published: 2017-01-10
Rated 5 out of 5 by from Produced beautiful immunoperoxidase apical Produced beautiful immunoperoxidase apical membrane staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Nice Western Blot data of V Nice Western Blot data of V-ATPase D expression in rat brain, mouse brain and human hippocampus tissue extracts and SK-N-SH whole cell lysate . -SCBT QC
Date published: 2013-07-03
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