Anti-V-ATPase C1 Antibody (G-5) is a mouse monoclonal IgG2b κ V-ATPase C1 antibody, cited in 4 publications, provided at 200 µg/ml
raised against amino acids 83-382 of V-ATPase C1 of human origin
Anti-V-ATPase C1 Antibody (G-5) is recommended for detection of V-ATPase C1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-V-ATPase C1 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V-ATPase C1 (G-5): sc-271077.
m-IgGκ BP-HRP is the preferred secondary detection reagent for V-ATPase C1 Antibody (G-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with V-ATPase C1 Antibody (G-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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V-ATPase C1 Antibody (G-5) is a high quality monoclonal V-ATPase C1 antibody (also designated V-ATPase C1 antibody) suitable for the detection of the V-ATPase C1 protein of mouse, rat and human origin. V-ATPase C1 Antibody (G-5) is available as both the non-conjugated anti-V-ATPase C1 antibody form, as well as multiple conjugated forms of anti-V-ATPase C1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c′ and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. V-ATPase C is an auxiliary subunit with ubiquitous expression. The gene encoding human V-ATPase C maps to chromosome 8q22.3. V-ATPase D is another auxiliary subunit.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 3 out of
Date published: 2020-06-19
Rated 5 out of
Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-04-29
Rated 5 out of
Endogenous levels of this protein detectedEndogenous levels of this protein detected using product sc-271077 by WB on 293T (non-transfected), HeLa and HepG2 whole cell lysates. -SCBT QC
Date published: 2015-04-02
Rated 5 out of
Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the mouse V-ATPase C1 protein via Western Blot analysis. -SCBT QC
Date published: 2015-03-06
Rated 5 out of
Positive detection of endogenous expression of VPositive detection of endogenous expression of V-ATPase C1 in SK-N-MC whole cell lysate, mouse kidney tissue extract and rat brain tissue extract. -SCBT QC
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