Anti-V-ATPase B1/2 Antibody (D-4) recommended for detection of V-ATPase B1 and V-ATPase B2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-V-ATPase B1/2 Antibody (D-4): sc-271832

Anti-V-ATPase B1/2 Antibody (D-4) is rated 5.0 out of 5 by 5.
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Datasheets

    • Anti-V-ATPase B1/2 Antibody (D-4) is a mouse monoclonal IgG1 κ V-ATPase B1/2 antibody, cited in 6 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 103-131 near the N-terminus of V-ATPase B1/2 of human origin
    • Anti-V-ATPase B1/2 Antibody (D-4) is recommended for detection of V-ATPase B1 and V-ATPase B2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-V-ATPase B1/2 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V-ATPase B1/2 (D-4): sc-271832.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for V-ATPase B1/2 Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with V-ATPase B1/2 Antibody (D-4) (see ordering information below).
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What application is the blocking peptide sc-271832 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody for WB application worked well at 1:250 dilution, the western blot signals are strong and specific
Date published: 2017-11-01
Rated 5 out of 5 by from Very good antibody The sample worked excellent with N2A total lysate. Primary 1:1000, secondary 1:10000, developed with Thermo western substrate, regular strength.
Date published: 2017-08-11
Rated 5 out of 5 by from Great monoclonal antibody V-ATPase B1/2 Antibody (D-4) showed strong band with very little background and nice IF and IHC stain
Date published: 2017-02-07
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Produced positive Western Blot data of V Produced positive Western Blot data of V-ATPase B1/2 expression in HEK293, SK-N-SH, MDBK and c4 whole cell lysates. -SCBT QC
Date published: 2014-11-26
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