Anti-V-ATPase B1/2 Antibody (D-4) is a mouse monoclonal IgG1 κ V-ATPase B1/2 antibody, cited in 6 publications, provided at 200 µg/ml
specific for an epitope mapping between amino acids 103-131 near the N-terminus of V-ATPase B1/2 of human origin
Anti-V-ATPase B1/2 Antibody (D-4) is recommended for detection of V-ATPase B1 and V-ATPase B2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-V-ATPase B1/2 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V-ATPase B1/2 (D-4): sc-271832.
m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for V-ATPase B1/2 Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with V-ATPase B1/2 Antibody (D-4) (see ordering information below).
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
V-ATPase B1/2 Antibody (D-4) is a high quality monoclonal V-ATPase B1/2 antibody (also designated VATB1 antibody, VATB2 antibody, H+ Transporting V1 Subunit antibody, ATPase H+ Transporting Lysosomal 56/58kDa V1 Subunit antibody, V-Type Proton ATPase Subunit B Brain Isoform antibody, Endomembrane Proton Pump 58 KDa Subunit antibody, Vacuolar Proton Pump Subunit antibody, V-ATPase Subunit antibody, ATP6B1 antibody, ATP6B2 antibody, VATB antibody, or V-ATPase B Subunit antibody) suitable for the detection of the V-ATPase B1/2 protein of mouse, rat and human origin. V-ATPase B1/2 Antibody (D-4) is available as both the non-conjugated anti-V-ATPase B1/2 antibody form, as well as multiple conjugated forms of anti-V-ATPase B1/2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c′ and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts. The gene encoding the human V-ATPase B2 isoform maps to chromosome 8p22-p21.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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