Anti-V-ATPase A1 Antibody (E-8) recommended for detection of V-ATPase A1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-V-ATPase A1 Antibody (E-8): sc-374475

Anti-V-ATPase A1 Antibody (E-8) is rated 4.6 out of 5 by 9.
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Datasheets

    • Anti-V-ATPase A1 Antibody (E-8) is a mouse monoclonal IgG2b κ V-ATPase A1 antibody, cited in 9 publications, provided at 200 µg/ml
    • raised against amino acids 71-210 mapping within a cytoplasmic domain of V-ATPase A1 of human origin
    • Anti-V-ATPase A1 Antibody (E-8) is recommended for detection of V-ATPase A1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine, porcine and canine
    • Anti-V-ATPase A1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V-ATPase A1 (E-8): sc-374475.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for V-ATPase A1 Antibody (E-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with V-ATPase A1 Antibody (E-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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The description for this antibody indicates it binds to the extracellular domain, but the papers it references points to the endogenous V0 subunit. Can this be clarified please?

Asked by: lucyh
Thank you for your question. This monoclonal antibody is raised against amino acids 71-210 mapping within a cytoplasmic domain of V-ATPase A1 of human origin. This information will be updated on our website.
Answered by: Technical Support
Date published: 2019-09-11

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Alternatively, we do offer V-ATPase A1 (E-8): sc-374475 monoclonal antibody directly conjugated to HRP, and a variety of fluorochromes, if you wish to omit using any secondary detection reagent and potential non-specific binding.
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 5 out of 5 by from worked great for murine samples worked great for murine samples for western blott.
Date published: 2019-11-18
Rated 5 out of 5 by from My Review for V-ATPase A1 Antibody Antibody works excellent for western blotting. Used at 1:500 in 5% BSA (overnight incubation). Detects a band of 150 kDa in cells lysed with non denaturing lysis buffer.
Date published: 2018-03-18
Rated 3 out of 5 by from Weak band We tried this antibody for WB in HEK293T cell lysates in 1:1000 dilution. We observed a weak band. Secondary antibody we used was anti-M IgG-680. The results may be improved by using M-IgGκ secondary antibodies.
Date published: 2017-11-14
Rated 5 out of 5 by from IHC detection We used this antibody by IHC to detect the expression of V-ATPase A1 in Human colon cancer tissue and normal tissue, V-ATPase was found high expression of V-ATPase A1 in colon cancer tissues, the positive result shows membrane and cytoplasm staining. The results obtained with this antibody are in line with our expectation of the experimental results. We suggest 1:200 dilution for IHC.
Date published: 2017-09-09
Rated 4 out of 5 by from Good antibody vATPase-A1 antibody detected vATPase in N2A cell lysates by western. Nonspecific bands were also present. Primary 1:1000, secondary 1:10000. Thermo West femto reagent was used for detection.
Date published: 2017-08-11
Rated 5 out of 5 by from great features V-ATPase A1 Antibody (E-8) produced strong and specific signal by WB, IHC and IF in 293t cells.
Date published: 2017-04-12
Rated 5 out of 5 by from Specific V-ATPase A1 Antibody (E-8) produced strong and specific signal by WB, IHC and IF
Date published: 2017-01-18
Rated 4 out of 5 by from Positive detection by WB using SK Positive detection by WB using SK-N-MC whole cell lysate. -SCBT QC
Date published: 2015-03-03
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