Anti-V-ATPase A1 Antibody (E-8) is a mouse monoclonal IgG2b κ V-ATPase A1 antibody, cited in 9 publications, provided at 200 µg/ml
raised against amino acids 71-210 mapping within a cytoplasmic domain of V-ATPase A1 of human origin
Anti-V-ATPase A1 Antibody (E-8) is recommended for detection of V-ATPase A1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine, porcine and canine
Anti-V-ATPase A1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V-ATPase A1 (E-8): sc-374475.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for V-ATPase A1 Antibody (E-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with V-ATPase A1 Antibody (E-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
V-ATPase A1 Antibody (E-8) is a high quality monoclonal V-ATPase A1 antibody (also designated ATP6V0A1 antibody, VPP1 antibody, ATPase H+ Transporting V0 Subunit A1 antibody, ATP6N1A antibody, ATP6N1 antibody, Stv1 antibody, VPP1 antibody, Vph1 antibody, A1 antibody, ATPase H+ Transporting Lysosomal Non-Catalytic Accessory Protein 1 (110/116kD) antibody, V-ATPase 116 KDa Subunit A1 antibody, or V-ATPase 116 KDa antibody) suitable for the detection of the V-ATPase A1 protein of mouse, rat and human origin. V-ATPase A1 Antibody (E-8) is available as both the non-conjugated anti-V-ATPase A1 antibody form, as well as multiple conjugated forms of anti-V-ATPase A1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The subunit of the vacuolar proton pump is a V-ATPase that has two different isoforms. The type I isoform contains an 18-base pair insert and is expressed in brain, whereas the truncated type II isoform is more widely expressed, including lung, kidney and spleen. The subunit of the vacuolar proton pump is located in clathrin-coated vesicles and is also found in osteoclasts. It consists of two fundamental domains, a hydrophilic amino-terminus, which has greater than 30% charged residues, and a hydrophobic carboxy terminus, which contains at least six transmembrane regions. The proton pump functions in coupling ATP hydrolysis by the cytoplasmic subunits to proton translocation by the intramembranous components of the pump. The inactivation of the osteoclast-specific vacuolar proton ATPase subunit is responsible for the lack of the enzyme in the apical membranes of osteoclast cells in osteosclerotic mutant mice, thus preventing the resorption function of these cells and leading to the osteopetrotic phenotype. The subunit, which co-localizes with the late endosomal marker Rab7 on vacuolar membranes, is essential for vacuole formation by selective swelling of late endosomes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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