Anti-V-ATPase A1 Antibody (E-8): sc-374475

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- Anti-V-ATPase A1 Antibody (E-8) is a mouse monoclonal IgG_2b κ V-ATPase A1 antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 71-210 mapping within a cytoplasmic domain of V-ATPase A1 of human origin
- Anti-V-ATPase A1 Antibody (E-8) is recommended for detection of V-ATPase A1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine, porcine and canine
- Anti-V-ATPase A1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 or Alexa Fluor^® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor^® 680 or Alexa Fluor^® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR^®Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V-ATPase A1 (E-8): sc-374475.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for V-ATPase A1 Antibody (E-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with V-ATPase A1 Antibody (E-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

SEE ALSO...

Additional V-ATPase Antibodies including V-ATPase α 1, V-ATPase B1, V-ATPase B2, V-ATPase C1, V-ATPase C2, V-ATPase D, V-ATPase D1, V-ATPase D2, V-ATPase G1 and V-ATPase G2
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

Anti-V-ATPase A1 Antibody (E-8) - Western Blotting - Image 356631

Anti-V-ATPase A1 Antibody (E-8) - Immunohistochemistry - Image 159084

Anti-V-ATPase A1 Antibody (E-8) - Immunofluorescence - Image 150754

Anti-V-ATPase A1 Antibody (E-8) - Western Blotting - Image 151051

Anti-V-ATPase A1 Antibody (E-8) - Western Blotting - Image 400395

Click on image or enlarge button to enlarge

V-ATPase A1 Antibody (E-8) is a high quality monoclonal V-ATPase A1 antibody (also designated ATP6V0A1 antibody, VPP1 antibody, ATPase H+ Transporting V0 Subunit A1 antibody, ATP6N1A antibody, ATP6N1 antibody, Stv1 antibody, VPP1 antibody, Vph1 antibody, A1 antibody, ATPase H+ Transporting Lysosomal Non-Catalytic Accessory Protein 1 (110/116kD) antibody, V-ATPase 116 KDa Subunit A1 antibody, or V-ATPase 116 KDa antibody) suitable for the detection of the V-ATPase A1 protein of mouse, rat and human origin. V-ATPase A1 Antibody (E-8) is available as both the non-conjugated anti-V-ATPase A1 antibody form, as well as multiple conjugated forms of anti-V-ATPase A1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The subunit of the vacuolar proton pump is a V-ATPase that has two different isoforms. The type I isoform contains an 18-base pair insert and is expressed in brain, whereas the truncated type II isoform is more widely expressed, including lung, kidney and spleen. The subunit of the vacuolar proton pump is located in clathrin-coated vesicles and is also found in osteoclasts. It consists of two fundamental domains, a hydrophilic amino-terminus, which has greater than 30% charged residues, and a hydrophobic carboxy terminus, which contains at least six transmembrane regions. The proton pump functions in coupling ATP hydrolysis by the cytoplasmic subunits to proton translocation by the intramembranous components of the pump. The inactivation of the osteoclast-specific vacuolar proton ATPase subunit is responsible for the lack of the enzyme in the apical membranes of osteoclast cells in osteosclerotic mutant mice, thus preventing the resorption function of these cells and leading to the osteopetrotic phenotype. The subunit, which co-localizes with the late endosomal marker Rab7 on vacuolar membranes, is essential for vacuole formation by selective swelling of late endosomes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Human	ATP6V0A1	535	17q21.2	XM_005257459, XM_005257461, XM_005257463, XM_011524908, XM_017024766, XM_017024767, XM_017024768, XM_017024769, XM_017024770, XM_017024771, XM_017024772, XM_017024773, XM_017024774, NM_001130020, NM_001130021, NM_005177	Q93050	192130
Mouse	Atp6v0a1	11975	11 D	XM_006532049, XM_006532050, XM_006532051, XM_006532052, XM_006532053, XM_006532054, XM_006532055, NM_001243049, NM_001243050, NM_001243051, NM_016920	Q9Z1G4	N/A

Full-length V-ATPase A1 protein sequence with immunogen highlighted:

                                        MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEKEIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFDEMADPDLLEESSSLLEPSEMGRGTPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQRVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPSILNRMQTNQTPPTYNKTNKFTYGFQNIVDAYGIGTYREINPAPYTIITFPFLFAVMFGDFGHGILMTLFAVWMVLRESRILSQKNENEMFSTVFSGRYIILLMGVFSMYTGLIYNDCFSKSLNIFGSSWSVRPMFTYNWTEETLRGNPVLQLNPALPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNHIYFKKPLNIYFGFIPEIIFMTSLFGYLVILIFYKWTAYDAHTSENAPSLLIHFINMFLFSYPESGYSMLYSGQKGIQCFLVVVALLCVPWMLLFKPLVLRRQYLRRKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHSEDADEPSEDEVFDFGDTMVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVIHIGLSVKSLAGGLVLFFFFTAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGTGFKFLPFSFEHIREGKFEE

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Protocols Online

The description for this antibody indicates it binds to the extracellular domain, but the papers it references points to the endogenous V0 subunit. Can this be clarified please?

Asked by: lucyh

Thank you for your question. This monoclonal antibody is raised against amino acids 71-210 mapping within a cytoplasmic domain of V-ATPase A1 of human origin. This information will be updated on our website.

Answered by: Technical Support

Date published: 2019-09-11

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Alternatively, we do offer V-ATPase A1 (E-8): sc-374475 monoclonal antibody directly conjugated to HRP, and a variety of fluorochromes, if you wish to omit using any secondary detection reagent and potential non-specific binding.

Answered by: Technical Support 7

Date published: 2017-01-25

Rated 5 out of 5 by woojonglee0917 from worked great for murine samples worked great for murine samples for western blott.

Date published: 2019-11-18

Rated 5 out of 5 by kras007 from My Review for V-ATPase A1 Antibody Antibody works excellent for western blotting. Used at 1:500 in 5% BSA (overnight incubation). Detects a band of 150 kDa in cells lysed with non denaturing lysis buffer.

Date published: 2018-03-18

Rated 3 out of 5 by Mumine from Weak band We tried this antibody for WB in HEK293T cell lysates in 1:1000 dilution. We observed a weak band. Secondary antibody we used was anti-M IgG-680. The results may be improved by using M-IgGκ secondary antibodies.

Date published: 2017-11-14

Rated 5 out of 5 by mengxing from IHC detection We used this antibody by IHC to detect the expression of V-ATPase A1 in Human colon cancer tissue and normal tissue, V-ATPase was found high expression of V-ATPase A1 in colon cancer tissues, the positive result shows membrane and cytoplasm staining. The results obtained with this antibody are in line with our expectation of the experimental results. We suggest 1:200 dilution for IHC.

Date published: 2017-09-09

Rated 4 out of 5 by Navneet from Good antibody vATPase-A1 antibody detected vATPase in N2A cell lysates by western. Nonspecific bands were also present. Primary 1:1000, secondary 1:10000. Thermo West femto reagent was used for detection.

Date published: 2017-08-11

Rated 5 out of 5 by fanko12 from great features V-ATPase A1 Antibody (E-8) produced strong and specific signal by WB, IHC and IF in 293t cells.

Date published: 2017-04-12

Rated 5 out of 5 by Professor Griffin from Specific V-ATPase A1 Antibody (E-8) produced strong and specific signal by WB, IHC and IF

Date published: 2017-01-18

Rated 4 out of 5 by LM from Positive detection by WB using SK Positive detection by WB using SK-N-MC whole cell lysate. -SCBT QC

Date published: 2015-03-03

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Anti-V-ATPase A1 Antibody (E-8): sc-374475

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SEE ALSO...

The description for this antibody indicates it binds to the extracellular domain, but the papers it references points to the endogenous V0 subunit. Can this be clarified please?

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?