Anti-USP14 Antibody (F-4) recommended for detection of USP14 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-USP14 Antibody (F-4): sc-398009

Anti-USP14 Antibody (F-4) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-USP14 Antibody (F-4) is a mouse monoclonal IgG1 κ USP14 antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 195-494 mapping at the C-terminus of USP14 of human origin
    • Anti-USP14 Antibody (F-4) is recommended for detection of USP14 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
    • Anti-USP14 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of USP14 (F-4): sc-398009.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for USP14 Antibody (F-4) for WB and IHC(P) applications. These reagents are now offered in bundles with USP14 Antibody (F-4) (see ordering information below).
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I did a blast using human USP14 against the Drosophila USP14, and the similarity between sequences is 55.3%. I would like to know if there would be a possibility of getting a small amount to test this antibody (USP14 Antibody (F-4): sc-398009) in flies.

Asked by: Anonymous
Thank you for your question. Please get in touch with our technical service department to arrange for a free sample of this antibody. Please be aware though, that sequence identity of 55% is very low for monoclonal antibodies and the product is very unlikely to recognize a protein with such a comparatively low level of sequence conservation.
Answered by: Technical Support Europe
Date published: 2020-10-21

The website only cites that "this antibody raised against amino acids 195-494 mapping at the C-terminus of USP14". I'm wondering whether the detailed amino acid targeting map of this monoclonal antibody is reachable?

Asked by: echo yan
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, USP14 (F-4): sc-398009, has not been determined.
Answered by: Technical Support
Date published: 2017-10-02

I am using USP14 (F-4): sc-398009 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Fine for WB Tested in SHSY-5Y human neuroblastoma cells (~15ug/lane), dilution 1:1000 in 3% milk in TBST. Got a single clear band at expected size.
Date published: 2018-05-15
Rated 5 out of 5 by from good I am able to detect endogenous USP14 in 293 cell lysates.
Date published: 2017-08-04
Rated 5 out of 5 by from Satisfactory Western Blot data of USP14 expression Satisfactory Western Blot data of USP14 expression in HeLa and MOLT-4 whole cell lysates. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Nice Western Blot data of USP14 expression in U Nice Western Blot data of USP14 expression in U-251-MG, SK-OV-3 and HCT-116 whole cell lysates. -SCBT QC
Date published: 2013-04-08
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