Anti-USP14 Antibody (A-1) recommended for detection of USP14 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-USP14 Antibody (A-1): sc-393872

Anti-USP14 Antibody (A-1) is rated 5.0 out of 5 by 3.
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Datasheets

    • USP14 Antibody (A-1) is a mouse monoclonal IgG2a κ, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 195-494 mapping at the C-terminus of USP14 of human origin
    • recommended for detection of USP14 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for USP14 Antibody (A-1) for WB applications. These reagents are now offered in bundles with USP14 Antibody (A-1) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with USP14 (A-1): sc-393872 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Very good Our lab good staining in IF(1:50) in HeLa cells showing cytoplasmic and nuclear localization.
Date published: 2017-08-31
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmic Beautiful immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Good Western Blot data of USP14 expression in HeLa Good Western Blot data of USP14 expression in HeLa, MOLT-4, U-251-MG, SK-OV-3 and HCT-116 whole cell lysates. -SCBT QC
Date published: 2013-07-13
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