Anti-USP14 Antibody (6E6) recommended for detection of USP14 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-USP14 Antibody (6E6): sc-100630

Anti-USP14 Antibody (6E6) is rated 4.3 out of 5 by 6.
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Datasheets

    • USP14 Antibody (6E6) is a mouse monoclonal IgG2a κ, cited in 7 publications, provided at 100 µg/ml
    • raised against recombinant USP14 of human origin
    • recommended for detection of USP14 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of USP14 (6E6): sc-100630.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for USP14 Antibody (6E6) for WB applications. These reagents are now offered in bundles with USP14 Antibody (6E6) (see ordering information below).
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I am using USP14 (6E6): sc-100630 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Antibody worked well for WB! I used this antibody for IF and WB. It worked well for both!
Date published: 2017-11-30
Rated 5 out of 5 by from Great antibody! Good choice if you're looking for an IF or WB antibody, strong and reliable.
Date published: 2017-01-23
Rated 4 out of 5 by from Clear band seen in WB in HeLa whole cell lysate Clear band seen in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-06
Rated 4 out of 5 by from Strong positive band detected in WB in both non Strong positive band detected in WB in both non-transfected and human USP14 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-11
Rated 4 out of 5 by from Published data for WB in HEK293T cells Published data for WB in HEK293T cells. -SCBT Publication Review
Date published: 2015-01-30
Rated 4 out of 5 by from Clear immunofluorescence nuclear staining Clear immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-01-17
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