uPA Antibody (H77A10)recommended for detection of uPA of mouse, rat and human origin by WB, IF, IHC(P) and ELISA

uPA Antibody (H77A10): sc-59727

uPA Antibody (H77A10) is rated 4.3 out of 5 by 7.
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Datasheets

    • uPA Antibody (H77A10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a recombinant protein corresponding to full-length wild-type (glycosylated) uPA of mouse origin
    • uPA Antibody (H77A10) is recommended for detection of uPA of mouse, rat and human origin by WB, IF, IHC(P) and ELISA
    • uPA Antibody (H77A10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of uPA (H77A10): sc-59727.
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Are there any issues with using sc-59727: uPA (H77A10) mouse monoclonal primary antibody on mouse samples?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-59727 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from great antibody I ordered this product month ago and used it for IF and Western blotting in cultured cells and skin tissues. It works very well for both in term of its specificity and sensitivity.
Date published: 2018-01-02
Rated 5 out of 5 by from The use of sc-59727(uPA) antibody Three weeks ago I purchased the antibody test equipment, the specificity of the antibody is excellent. WB strip is very clear, and our research group has purchased the antibody.
Date published: 2017-09-13
Rated 5 out of 5 by from good quanlities! WB on HCC cells, dilute in 1:500, show specific and clearly bands.
Date published: 2017-05-03
Rated 1 out of 5 by from No bands seen Western blotting was performed using twelve different mouse osteosarcoma cell lines which were previously confirmed to express uPA gene based on RT-PCR. Conditions: 12% gel electrophoresis, wet transfer over 2 hours, primary antibodies at 1:200 dilution in 5% mild blocking solution (4C overnight). There was no band visible following the protocol, although positive control (b-actin) was positive.
Date published: 2017-03-08
Rated 5 out of 5 by from perfect result I have used this antibody to detect this protein in mouse lung tumors. It works well in IHC and western blot.
Date published: 2017-01-06
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