Anti-UBE2F Antibody (C-11) recommended for detection of UBE2F of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-UBE2F Antibody (C-11): sc-398668

Anti-UBE2F Antibody (C-11) is rated 4.2 out of 5 by 5.
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Datasheets

    • Anti-UBE2F Antibody (C-11) is a mouse monoclonal IgG1 κ UBE2F antibody, cited in 3 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 118-140 within an internal region of UBE2F of human origin
    • Anti-UBE2F Antibody (C-11) is recommended for detection of UBE2F of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-UBE2F Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of UBE2F (C-11): sc-398668.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UBE2F Antibody (C-11) for WB applications. These reagents are now offered in bundles with UBE2F Antibody (C-11) (see ordering information below).
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What application is the blocking peptide sc-398668 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from review for UBE2F antibody We bought and used this antibody, and it works in our lab!
Date published: 2019-11-26
Rated 5 out of 5 by from good western blot! The western blot bands is very good in A498 cells!!
Date published: 2017-11-21
Rated 4 out of 5 by from UBE2F Although it needs longer exposure to see band, the band is nice and specific.
Date published: 2017-10-06
Rated 3 out of 5 by from Getting weak bands Getting weak bands, not sure if it is the protein level, but so far antibody is working
Date published: 2015-03-13
Rated 5 out of 5 by from Good Western blot data of UBE2F expression in non Good Western blot data of UBE2F expression in non-transfected 293T, mouse UBE2F transfected 293T Jurkat, HeLa, HL-60 and NTERA-2 cl.D1 whole cell lysates. -SCBT QC
Date published: 2013-04-06
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