UBE2C siRNA and shRNA Plasmids (m)

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- Target species: mouse; for corresponding human product, see UBE2C siRNA and shRNA Plasmids (h)
- Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
- For independent verification of UBE2C gene silencing results, individual duplex components or plasmids are also available upon request
- Suitable control antibody: UBE2C Antibody (B-12): sc-166339 is recommended as control antibody for monitoring of UBE2C expression knockdown by Western blotting or immunofluorescence
- Get details about si/shRNA Gene Silencers
- Gene silencers are intended for noncommercial and research use only. Click here for details.

Ordering Information
siRNA
Primer

Gene Info

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Mouse	Ube2c	68612	2 H3	NM_026785	Q9D1C1	N/A

Description

10 µM, 50-100 transfections
siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
for independent verification of UBE2C gene silencing results, individual siRNA duplex components are also available upon request
suitable control antibody: UBE2C Antibody (B-12): sc-166339
sqRT-PCR Primer also available: UBE2C (m)-PR: sc-61743-PR, 20 µl
Get details about si/shRNA Gene Silencers

See Protocol Online.

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Description

20 µl of each primer set is provided at a concentration of 10 µM
UBE2C Primer (m) includes A set (forward & reverse) and B set (forward & reverse) primers
Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR

See Protocol Online.

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Description

20 µg, up to 20 transfections
shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
shRNA sequences correspond to UBE2C siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
shRNA Plasmids are intended only for non-commercial use.
suitable control antibody: UBE2C Antibody (B-12): sc-166339
sqRT-PCR Primer also available: UBE2C (m)-PR: sc-61743-PR, 20 µl

See Protocol Online.

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Description

200 µl viral stock containing 1x 10⁶ infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
shRNA sequences correspond to UBE2C siRNA Gene Silencer sequences
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
Lentiviral Particles are intended for non-commercial use.

See Protocol Online.

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请问shRNA质粒的图谱是什么？质粒能否扩增

Asked by: NOST

Thank you for your question.We can provide you with a general Plasmid vector map prior to purchase. Please contact our Technical Service Department to request this information.Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website. We do not support customers culturing or amplifying the plasmids themselves in bacteria. They are intended to be ready-to-use consumable products.

Answered by: Technical Support 9

Date published: 2019-01-09

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UBE2C siRNA and shRNA Plasmids (m)

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Gene Info

请问shRNA质粒的图谱是什么？质粒能否扩增