Anti-UBE2C Antibody (B-12) recommended for detection of UBE2C isoforms 1, 2, 3 and 5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-UBE2C Antibody (B-12): sc-166339

Anti-UBE2C Antibody (B-12) is rated 4.5 out of 5 by 4.
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Datasheets

    • UBE2C Antibody (B-12) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1-25 at the N-terminus of UBE2C of human origin
    • Anti-UBE2C Antibody (B-12) is recommended for detection of UBE2C isoforms 1, 2, 3 and 5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-UBE2C Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of UBE2C (B-12): sc-166339.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UBE2C Antibody (B-12) for WB and IHC(P) applications. These reagents are now offered in bundles with UBE2C Antibody (B-12) (see ordering information below).
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What application is the blocking peptide sc-166339 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from WB There was no signal in human macrophage lysate by WB.
Date published: 2017-04-19
Rated 5 out of 5 by from Strong band observed in WB in human UBE2C Strong band observed in WB in human UBE2C transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-06-04
Rated 5 out of 5 by from Clear Clear, specific band obtained in WB in human UBE2C transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic staining Great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-03-04
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