UBC8 Antibody (E-5): sc-166276
- UBC8 Antibody (E-5) is a mouse monoclonal IgG2a κ UBC8 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 11-42 near the N-terminus of UBC8 of mouse origin
- UBC8 Antibody (E-5) is recommended for detection of UBC8 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-UBC8 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for UBC8 Antibody (E-5). This work is in progress.
UBC8 Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects UBC8 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBC8 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBC8, also known as ubiquitin-conjugating enzyme 8, plays a crucial role in the ubiquitin-proteasome pathway, which is essential for maintaining cellular homeostasis by regulating protein degradation. UBC8 is primarily located in the cytoplasm and is particularly enriched in the central nervous system, where UBC8 is involved in the degradation of misfolded or damaged proteins, thereby preventing the accumulation of potentially toxic aggregates. UBC8 interacts with several key proteins, including Parkin, a RING-finger protein associated with familial Parkinson′s disease, and other E3 ligases such as the human homolog of Drosophila ariadne (HHARI) and UBC7-associated protein (H7-AP1). UBC8′s ability to form thioester bonds with ubiquitin in an E1-dependent manner is vital for tagging substrates for proteasomal degradation, highlighting UBC8′s importance in cellular quality control mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBC8 Antibody (E-5) References:
- The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. | Moynihan, TP., et al. 1999. J Biol Chem. 274: 30963-8. PMID: 10521492
- Parkin suppresses unfolded protein stress-induced cell death through its E3 ubiquitin-protein ligase activity. | Imai, Y., et al. 2000. J Biol Chem. 275: 35661-4. PMID: 10973942
- Parkin functions as an E2-dependent ubiquitin- protein ligase and promotes the degradation of the synaptic vesicle-associated protein, CDCrel-1. | Zhang, Y., et al. 2000. Proc Natl Acad Sci U S A. 97: 13354-9. PMID: 11078524
- Characterisation of the human and mouse orthologues of the Drosophila ariadne gene. | Tan, NG., et al. 2000. Cytogenet Cell Genet. 90: 242-5. PMID: 11124525
- A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. | Niwa, J., et al. 2001. Biochem Biophys Res Commun. 281: 706-13. PMID: 11237715
- Ubiquitin, proteasomes, and the regulation of intracellular protein degradation. | Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
- The ubiquitin-proteasome proteolytic pathway. | Ciechanover, A. 1994. Cell. 79: 13-21. PMID: 7923371
- The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins. | Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
- cDNA cloning, characterization, and chromosome mapping of UBE2E2 encoding a human ubiquitin-conjugating E2 enzyme. | Kimura, M., et al. 1997. Cytogenet Cell Genet. 78: 107-11. PMID: 9371400
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBC8 Antibody (E-5) | sc-166276 | 200 µg/ml | $322.00 | |||
UBC8 Antibody (E-5) AC | sc-166276 AC | 500 µg/ml, 25% agarose | $424.00 | |||
UBC8 Antibody (E-5) HRP | sc-166276 HRP | 200 µg/ml | $322.00 | |||
UBC8 Antibody (E-5) FITC | sc-166276 FITC | 200 µg/ml | $336.00 | |||
UBC8 Antibody (E-5) PE | sc-166276 PE | 200 µg/ml | $349.00 | |||
UBC8 Antibody (E-5) Alexa Fluor® 488 | sc-166276 AF488 | 200 µg/ml | $364.00 | |||
UBC8 Antibody (E-5) Alexa Fluor® 546 | sc-166276 AF546 | 200 µg/ml | $364.00 | |||
UBC8 Antibody (E-5) Alexa Fluor® 594 | sc-166276 AF594 | 200 µg/ml | $364.00 | |||
UBC8 Antibody (E-5) Alexa Fluor® 647 | sc-166276 AF647 | 200 µg/ml | $364.00 | |||
UBC8 Antibody (E-5) Alexa Fluor® 680 | sc-166276 AF680 | 200 µg/ml | $364.00 | |||
UBC8 Antibody (E-5) Alexa Fluor® 790 | sc-166276 AF790 | 200 µg/ml | $364.00 | |||
UBC8 (E-5) Neutralizing Peptide | sc-166276 P | 100 µg/0.5 ml | $69.00 |