Anti-TSHR Antibody (4C1) recommended for detection of TSHR of human origin by IP, IF and FCM

Anti-TSHR Antibody (4C1): sc-32262

Anti-TSHR Antibody (4C1) is rated 4.0 out of 5 by 4.
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Datasheets

    • TSHR Antibody (4C1) is a mouse monoclonal IgG2a κ, cited in 6 publications, provided at 200 µg/ml
    • raised against an extracellular domain of TSHR of human origin, epitope mapping to amino acids 381-384
    • recommended for detection of TSHR of human origin by IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for TSHR Antibody (4C1) for WB and IHC(P) applications. These reagents are now offered in bundles with TSHR Antibody (4C1) (see ordering information below).
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How is this mAb expressed? Hybridoma? is the sequence known?

Asked by: R F Z
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801. ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-10-18

Can TSHR (4C1): sc-32262 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TSHR (4C1): sc-32262 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didn't work for IF staining of cryosections The antibody did not work for cryo sections of primary human thyroid tissue.
Date published: 2019-02-08
Rated 5 out of 5 by from positive staining liver and lipid tissue I used the antibody 3 months ago. We used it evaluate the TSHR expression in thyroid, liver and lipid tissue. With the dilution of 1:500, the band is good in Western blot analysis.
Date published: 2016-12-08
Rated 5 out of 5 by from Excellent intracellular FCM data of fixed Excellent intracellular FCM data of fixed and permeabilized HeLa cells. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Nice indirect FCM data of GPI Nice indirect FCM data of GPI-anchored TSHR (Da Costa CR, Johnstone AP. -SCBT QC
Date published: 2014-08-06
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