Anti-TSHR Antibody (2C11) recommended for detection of TSHR of human origin by WB, IP, IF, IHC(P) and FCM

Anti-TSHR Antibody (2C11): sc-32263

Anti-TSHR Antibody (2C11) is rated 4.0 out of 5 by 4.
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Datasheets

    • TSHR Antibody (2C11) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
    • raised against human recombinant GST-TSHR fusion protein, with epitope mapping to amino acids 352-362
    • recommended for detection of TSHR of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TSHR Antibody (2C11) for WB and IHC(P) applications. These reagents are now offered in bundles with TSHR Antibody (2C11) (see ordering information below).
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Can TSHR (2C11): sc-32263 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TSHR (2C11): sc-32263 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Used for IP with L Used for IP with L-02, human and rat liver, & human and rat thyroid samples. -SCBT Publication Review
Date published: 2015-06-23
Rated 4 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC
Date published: 2015-02-09
Rated 4 out of 5 by from Successfully immunoprecipitated TSHR protein Successfully immunoprecipitated TSHR protein from human COS-7 cells. -SCBT Publication Review
Date published: 2015-01-21
Rated 4 out of 5 by from Produced good indirect FCM data for GPI Produced good indirect FCM data for GPI-anchored TSHR (Da Costa CR, Johnstone AP. -SCBT QC
Date published: 2013-12-19
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