Anti-TSG-6 Antibody (E-1) recommended for detection of precursor and mature chain of TSG-6 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-TSG-6 Antibody (E-1): sc-377277

Anti-TSG-6 Antibody (E-1) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-TSG-6 Antibody (E-1) is a mouse monoclonal IgG2b κ TSG-6 antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 135-277 mapping at the C-terminus of TSG-6 of human origin
    • TSG-6 Antibody (E-1) is recommended for detection of precursor and mature chain of TSG-6 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TSG-6 Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TSG-6 (E-1): sc-377277.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for TSG-6 Antibody (E-1) for WB and IHC(P) applications. These reagents are now offered in bundles with TSG-6 Antibody (E-1) (see ordering information below).
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Can TSG-6 (E-1): sc-377277 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TSG-6 (E-1): sc-377277 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked Really well Incubated this antibody 1:100 dilution overnight, was able to clearly detect in 5 min exposure. a little bit of nonspecific binding though that I can't get rid of.
Date published: 2017-08-18
Rated 5 out of 5 by from suit to IHC-P some macrophages were positive for tnfaip6 with this antibody in doing IHC-P
Date published: 2016-12-12
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic staining Superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Great Western Blot data of TSG Great Western Blot data of TSG-6 expression in non-transfected and human TSG-6 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-11-19
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