Anti-TRPC6 Antibody (B-10) recommended for detection of TRPC6 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-TRPC6 Antibody (B-10): sc-515837

Anti-TRPC6 Antibody (B-10) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-TRPC6 Antibody (B-10) is a mouse monoclonal IgG1 κ TRPC6 antibody, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 546-590 mapping within an extracellular domain of TRPC6 of human origin
    • Anti-TRPC6 Antibody (B-10) is recommended for detection of TRPC6 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-TRPC6 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TRPC6 (B-10): sc-515837.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRPC6 Antibody (B-10) for WB applications. These reagents are now offered in bundles with TRPC6 Antibody (B-10) (see ordering information below).
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How much sample do you recommend loading on the gel for Western blot detection using TRPC6 (B-10): sc-515837?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from good result on WB The TRPC6 antibody works well in Werstern Blot detection using Rat articular chondrocytes cell at 1:300. The protein size locates at 90-110kD . we got a clear protein band.
Date published: 2017-07-14
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