TRPC3/6/7 Double Nickase Plasmid (h): sc-403740-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRPC3/6/7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TRPC3/6/7 Double Nickase Plasmid (h) and TRPC3/6/7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TRPC3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRPC6 Antibody (B-10): sc-515837
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRPC3/6/7 Double Nickase Plasmid (h) | sc-403740-NIC | 20 µg | $410.00 | |||
TRPC3/6/7 Double Nickase Plasmid (h2) | sc-403740-NIC-2 | 20 µg | $410.00 |
TRPC3 encodes a diacylglycerol-activated, nonselective cation channel of the TRPC subfamily that contributes to receptor-operated Ca2+ entry and membrane depolarization. TRPC3 forms functional assemblies with related TRPC6 and TRPC7 subunits, linking GPCR and receptor tyrosine kinase signaling to calcium-dependent transcriptional programs, cytoskeletal remodeling, and contractile responses. Channel activity is commonly engaged downstream of PLCβ/PLCγ pathways and modulated by lipid signaling, kinase cascades, and cellular redox state. Dysregulated TRPC3/6/7 signaling has been implicated in pathological remodeling and excitability across multiple tissues, including cardiovascular, renal, and nervous systems, making it a useful node for studying calcium-driven stress and differentiation pathways.
TRPC3/6/7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TRPC3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TRPC3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TRPC3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TRPC3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.