Troponin C slow skeletal/cardiac Antibody (1A2)recommended for detection of Troponin C slow skeletal/cardiac of human origin by WB and IP

Troponin C slow skeletal/cardiac Antibody (1A2): sc-52264

Troponin C slow skeletal/cardiac Antibody (1A2) is rated 5.0 out of 5 by 3.
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Datasheets

    • Troponin C slow skeletal/cardiac Antibody (1A2) is a mouse monoclonal IgG2a provided at 100 µg/ml
    • raised against slow skeletal isoform of Troponin C of human origin
    • recommended for detection of Troponin C slow skeletal/cardiac of human origin by WB and IP
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I am using Troponin C slow skeletal/cardiac (1A2): sc-52264 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Well done in WB We detected positive signal in WB using heart tissue of mouse.
Date published: 2016-11-16
Rated 5 out of 5 by from Strong band in WB in human Troponin C transfected Strong band in WB in human Troponin C transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Good Good, specificity in WB in SJRH30, HISM and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-04
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