Troponin C fast skeletal Antibody (E-7)recommended for detection of fast skeletal isoform of Troponin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Troponin C fast skeletal Antibody (E-7): sc-48347

Troponin C fast skeletal Antibody (E-7) is rated 4.6 out of 5 by 5.
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Datasheets

    • Troponin C fast skeletal Antibody (E-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 51-160 of Troponin C of human origin
    • Troponin C fast skeletal Antibody (E-7) is recommended for detection of fast skeletal isoform of Troponin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Troponin C fast skeletal Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Troponin C fast skeletal (E-7): sc-48347.
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I am using Troponin C fast skeletal (E-7): sc-48347 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Publishable data in IF using monkey EB's Publishable data in IF using monkey EB's. -SCBT Publication Review
Date published: 2015-04-30
Rated 5 out of 5 by from Strong band seen in WB using human recombinant Strong band seen in WB using human recombinant Troponin C. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skeletal muscle tissue. -SCBT QC
Date published: 2015-01-27
Rated 4 out of 5 by from Publishable data in WB using mouse gubernaculum Publishable data in WB using mouse gubernaculum cells. -SCBT Publication Review
Date published: 2015-01-18
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