Tris hydrochloride solution, pH 7.8Non-sterile; 0.2 µm filtered, 1M stock solution used to make buffers

Tris hydrochloride solution, pH 7.8 (CAS 1185-53-1 (solid))

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Synonym: Tris(hydroxymethyl)aminomethane hydrochloride solution, pH 7.8
Application: Non-sterile; 0.2 µm filtered, 1M stock solution used to make buffers
CAS Number: 1185-53-1 (solid)
Molecular Weight: 157.60
Molecular Formula: NH2C(CH2OH)3 · HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
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pH 7.8, 1 M. Tris hydrochloride has been used as a component of extraction buffer e.g. phenol extraction of DNA or RNA; and as a component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE. The pH values of Tris buffer solutions are temperature and concentration-dependent. Between 5 °C and 25 °C, the pH value increases an average of 0.03 pH units for each °C decrease in temperature. As the buffer temperature increases from 25 °C to 37 °C, the pH value decreases an average of 0.025 pH units per °C. Increasing the concentration of Tris from 0.05 M to 0.5M will increase the pH value by about 0.05 units. Decreasing the concentration from 0.05 M to 0.005 M will decrease the pH value by about 0.05 units.

Formulation :
1 M
Physical State :
Liquid
Solubility :
Soluble in water.
pH :
7.8
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
2
PubChem CID :
93573
MDL Number :
MFCD00012590
EC Number :
214-684-5
Beilstein Registry :
3675235
SMILES :
C(C(CO)(CO)N)O.Cl

Download SDS (MSDS)

Certificate of Analysis

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