Anti-TREM-2 Antibody (B-3) recommended for detection of TREM-2 isoforms 1-3 of human origin by WB, IP, IF and ELISA

Anti-TREM-2 Antibody (B-3): sc-373828

Anti-TREM-2 Antibody (B-3) is rated 4.8 out of 5 by 12.
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Datasheets

    • Anti-TREM-2 Antibody (B-3) is a mouse monoclonal IgG1 κ TREM-2 antibody, cited in 19 publications, provided at 200 µg/ml
    • raised against amino acids 1-160 mapping at the N-terminus of TREM-2 of human origin
    • TREM-2 Antibody (B-3) is recommended for detection of TREM-2 isoforms 1-3 of human origin by WB, IP, IF and ELISA
    • Anti-TREM-2 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TREM-2 (B-3): sc-373828.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TREM-2 Antibody (B-3) for WB applications. These reagents are now offered in bundles with TREM-2 Antibody (B-3) (see ordering information below).
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您好,亲爱的老师!麻烦问一下您,sc-373828可以做c57小鼠的免疫组织化学分析吗?因为动物实验需要,谢谢!

Asked by: sophia339
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We suggest this product on frozen tissue for IHC application. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。sc-373828推荐应用于冰冻切片的免疫组织化学实验,不推荐石蜡包埋样本的免疫组织化学实验。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-02-19

do you have a biotinylated version (or carrier-free) of this antibody (anti-TREM2, B-3)

Asked by: Rishi
Thank you for the question. Our special orders dept. <specialorders @scbt.com> will contact you by email to learn about your requirements.
Answered by: Technical Support Europe
Date published: 2018-01-31

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Germaine
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-09
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Rated 5 out of 5 by from 可以很好检测出TREM2 几个月前获得小样,跑Western Blot,实验很成功,没有杂带,一抗可以重复用好几次,都没有问题的
Date published: 2020-10-19
Rated 5 out of 5 by from Works The antibody works as intended. Tested multiple times.
Date published: 2018-10-11
Rated 5 out of 5 by from Good antibody IF (1:200) on Cryosection from 4%PFA fixed E13.5 mouse cerebellum, specific signal were detected.
Date published: 2017-11-08
Rated 5 out of 5 by from good result when we got the antibody,we did some pretest,by adjust the conditions such as antibody dilution ,sample loading amount, block reagents ,expose time and so on.We recommend use 1:400 to 1:1000 dilution ,but not more than 1:1000, 5% BSA maybe more better.
Date published: 2017-07-15
Rated 5 out of 5 by from Beautiful data Great antibody data in Western blot, show a strong band
Date published: 2017-05-19
Rated 5 out of 5 by from Excellent Reacts well for western blot applications strong signal
Date published: 2017-01-06
Rated 5 out of 5 by from Western blot good result Western Blot data published in human brain samples.
Date published: 2016-12-23
Rated 5 out of 5 by from Good WB publishable data gathered with mouse Good WB publishable data gathered with mouse microglial cell line. -SCBT Publication Review
Date published: 2015-06-17
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