Anti-Trehalase Antibody (D-2) recommended for detection of Trehalase of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Trehalase Antibody (D-2): sc-390034

Anti-Trehalase Antibody (D-2) is rated 3.7 out of 5 by 3.
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Datasheets

    • Anti-Trehalase Antibody (D-2) is a mouse monoclonal IgG1 κ Trehalase antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 181-405 mapping within an internal region of Trehalase of human origin
    • Anti-Trehalase Antibody (D-2) is recommended for detection of Trehalase of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Trehalase Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Trehalase (D-2): sc-390034.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Trehalase Antibody (D-2) for WB applications. These reagents are now offered in bundles with Trehalase Antibody (D-2) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with Trehalase (D-2): sc-390034 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 1 out of 5 by from IHC Assay Review for Trehalase mAB Application: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from Produced exceptional immunofluorescence membrane Produced exceptional immunofluorescence membrane staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-04-04
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