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TRB-3 (tribbles 3), also called NIPK (neuronal cell death-inducible protein kinase) disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRB3 binds to ATF4, inhibiting its transcriptional activation activity, and regulates activation of MAP kinases. In the liver, TRB-3 is a target for PPAR-alpha. Amounts of TRB3 RNA and protein are higher in livers of diabetic mice compared with those in wildtype mice. TRB3 contributes to insulin resistance in individuals with susceptibility to type II diabetes. Highest expression of TRB-3 is in liver, pancreas, peripheral blood leukocytes and bone marrow.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
what is the molecular weight of TRB-3 in Western blot.
Asked by: tieniu
Thank you for your question. The expected height at which TRB-3 Antibody (G-10): sc-271572 detects TRB-3 is 45 kDa.
Answered by: Tech Support Europe
Date published: 2019-08-26
What is the recommended starting dilution for Western blot for sc-271572: TRB-3 (G-10) monoclonal antibody?
Asked by: jenni
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
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